Composite

Part:BBa_K4192114:Design

Designed by: Lehan Dong   Group: iGEM22_CAU_China   (2022-09-29)


Plac-RBS-cdg-RBS-gacA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 468
    Illegal BamHI site found at 459
    Illegal XhoI site found at 557
    Illegal XhoI site found at 1001
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 392
    Illegal NgoMIV site found at 1489
    Illegal AgeI site found at 959
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 410
    Illegal BsaI.rc site found at 977


Design Notes

This part cannot be used for Escherichia coli. At present, it is only tested in Pseudomonas fluorescens 2P24. This part has the best effect when the environmental calcium ion concentration is about 0.01M.



Source

Plac comes from plasmid pBBR1MCS-2. The rbs sequence (BBa_K4192000) is synthetic. The cdg and gacA come from the genome of Pseudomonas fluorescens 2P24.

References