![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4192114:Design
Designed by: Lehan Dong Group: iGEM22_CAU_China (2022-09-29)
Plac-RBS-cdg-RBS-gacA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 468
Illegal BamHI site found at 459
Illegal XhoI site found at 557
Illegal XhoI site found at 1001 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 392
Illegal NgoMIV site found at 1489
Illegal AgeI site found at 959 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 410
Illegal BsaI.rc site found at 977
Design Notes
This part cannot be used for Escherichia coli. At present, it is only tested in Pseudomonas fluorescens 2P24. This part has the best effect when the environmental calcium ion concentration is about 0.01M.
Source
Plac comes from plasmid pBBR1MCS-2. The rbs sequence (BBa_K4192000) is synthetic. The cdg and gacA come from the genome of Pseudomonas fluorescens 2P24.